Document Details
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Document Type |
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Thesis |
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Document Title |
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EFFECT OF LOCAL MANGROVE LEAVES EXTRACT ON DIABETES INDUCED BY STREPTOZOTOCIN IN MALE RATS تأثير مستخلص أوراق المانجروف المحلي على داء السكري المستحدث بواسطة الستربتوزوتوسين في ذكور الجرذان |
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Subject |
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faculty of science |
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Document Language |
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Arabic |
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Abstract |
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Diabetes mellitus is a metabolic disorder which affects whole body systems. Currently, different anti-diabetic drugs are used to control the disease. Most of these synthetic drugs are associated with many side effects. Medicinal plants attract growing interest in the therapeutic management of diabetes mellitus which is still a major challenge. Mangroves are ecologically important trees with an affinity for the saline parts of the world. In Saudi Arabia, Avicenna marina grows from latitude 27° 25’N down to below Jizan, accompanied by some Rhizophora mucronata trees on few sites. The present study is the first detailed experimental research designed to assess the effect of aqueous extract of R. mucronata, A. marina and their mixture on streptozotocin-induced diabetic rats. In this study, 120 male albino rats were equally allocated into 8 groups. Rats of the first group (G1) served as normal controls. Rats of the second group (G2) were intraperitoneally injected with a single dose (60 mg/kg body weight of streptozotocin. The diabetic rats of the third, fourth and fifth groups (G3-G5) were treated with the extracts of R. mucronata (400 mg/kg body weight), A. marina (400 mg/kg body weight and a mixture of R. mucronata (200 mg/kg body weight) and A. marina (200 mg/kg body weight), respectively. The non-diabetic rats of the sixth, seventh and eighth groups (G6-G8) were administered extracts of R. mucronata, A. marina and their mixture, respectively. After six weeks of treatment, the clinical signs, biochemical, histological and immunohistochemical changes were investigated. Polyuria, hyperglycemia, glucosuria, body weight loss, elevation of serum glucose, liver enzymes, total bilirubin, creatinine, blood urea nitrogen, uric acid, triglycerides, cholesterol, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol and tissue malondialdehyde and a decrease in the serum insulin, total protein, albumin, globulin, high density lipoprotein-cholesterol, catalase, reduced glutathione and superoxide dismutase levels were observed in streptozotocin-induced diabetic rats. Administration of mangrove plant extracts to diabetic rats significantly amended the studied parameters. The histopathological examination of pancreas in streptozotocin-induced diabetic rats revealed degenerative, apoptotic and necrotic changes in beta cells of islets of Langerhans. It was observed that administration of plant extracts to diabetic rats caused a notable increase in islet cell population and protection of the pancreas, kidney, liver and testis against the damaging effect of hyperglycemia. Immunohistochemical investigation of pancreas showed about 65-75 %/high power field of the beta cells with a positive deep brownish cytoplasmic and nuclear stainability for the apoptotic marker caspase-3 and about 10-35%/high power field positively reacted and stained beta cells with the insulin diabetic bio-marker. Also, liver, kidney and testes sections showed variable degrees of changes. In addition, non-diabetic rats, who were administered the plant extracts, revealed non-significant changes, as compared to normal control group. The results of the present study showed that the plant extracts showed anti-hyperglycemic, insulinotropic, anti-hyperlipidemic and anti-oxidant actions. Therefore, the findings of this investigation are important for pharmaceutical industry and scientific research for the development of more preventive and effective therapeutic plant-derived anti-diabetic drugs. |
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Supervisor |
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Dr. Issam only who |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Added Date |
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Thursday, March 12, 2020 |
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Researchers
| عبيدالله حمدان الجقثمي | Al-jaghthmi, Obidallah Hamdan | Researcher | Doctorate | |
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