Document Details

Document Type : Thesis 
Document Title :
IN VIVO BIODISTRIBUTION AND TOXICITY ASSESSMENT OF MULTIFUNCTIONAL MAGNETIC NANOPARTICLES
دراسة داخلية لتقييم التوزيع الحيوى والسمية لجزيئات النانو المغناطيسية متعددة الوظائف
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : This work aims to study in vivo biodistribution and toxicity of Curcumin coated iron oxide nanoparticles (Cur-IONPs) on different body organs. The physicochemical properties of the prepared Cur-IONPs were investigated using: High resolution transmission electron microscope, particle size distribution, zeta potential and vibrating sample magnetometer. For the in vivo study, two treatment protocols were designed: single dose administration (5 mg/ kg) of Cur-IONPs and repeated doses administration (6 doses/2 weeks, 5mg/kg) of Cur-IONPs. To assess the biodistribution and toxicity of Cur-IONPs, experiments were conducted including: (i) atomic absorption spectroscopy measuring iron content in the liver, kidney, spleen and brain, (ii) serum biochemical analysis assessing the performance of these organs, (iii) histopathological examination investigating the cellular integrity of these organs, and (ix) body weight measurements. The integrated in vivo results demonstrated that Cur-IONPs were mostly taken up in the liver and spleen reaching their highest levels on days 1 and 2, respectively for the single dose protocol. In the repeated dose protocol maximum iron accumulation in liver and spleen was reached at 3 days post experiment termination. For the brain, the results showed significant accumulation of Cur-IONPs at 1 h to 1 day post injection in the first protocol, whereas it reached maximum at 3 days post experiment termination in the second protocol. This represents the successful penetration Cur-IONPs across the blood-brain barrier. Serum biochemical analysis for all the experimental groups demonstrated a temporal disturbance in the performance of body organs. Histopathological examination, from the two protocols, revealed normal morphological shape with no abnormalities or defects indicating the integrity of the cells. Also, the body weights for both protocols showed no alteration throughout the experiment. It has been deduced that the promising green synthesized Cur-IONPs as an “All in One” nanoplatform is safe enough to be used in diagnosis and therapy. 
Supervisor : Dr. Samia Aboushoushah 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Dr. Nihal Elbialy 
Added Date : Monday, March 25, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
وفاء وقيان الشمريAlshammari, Wafa WuqayyanResearcherMaster 

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