|
Document Type |
: |
Thesis |
|
Document Title |
: |
Antioxidant Activity of Anticancer Chromium and Cobalt Complexes of bromobenzaldehyde-iminacetophenone Against Ehrlich Ascites Carcinoma Cells Induced in Mice النشاط المضاد للأكسدة لمتراكبات الكروم والكوبالت لبروموبنزألدهيد-أيمين الأسيتوفينون المضادة للسرطان ضد خلايا ايرلخ السرطانية المستحدثة في الفئران |
|
Subject |
: |
Faculty of Sciences |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
This study was carried out to compare the antibacterial, antitumor and antioxidant activities of cobalt and chromium complexes of bromobenzaldehyde -iminacetophenone. In vitro antibacterial activities of the synthesized complexes were tested against two bacterial strains; Staphylococcus aureus and Escherchia coli. HepG2, A549 and PC3 carcinoma cell lines were used for evaluating in vitro cytotoxicity of the complexes. For in vivo study, 150 male albino mice were divided into six main groups: normal, EAC, Co - complex, Cr - complex, Co - complex treated (40mg/ Kg body weight) and Cr- complex treated (70mg/ Kg body weight) groups. Results indicated that both complexes have the capacity to inhibit the metabolic growth of the tested microorganisms, with higher activity for cobalt complex than chromium complex. Both complexes demonstrated in vitro medium cytotoxicity against tested cell lines. Co- complex had lower IC50 value against PC3 cell line ( 21.7 µg/ ml ), while Cr- complex showed lower IC50 values against HepG2 (12.2 µg/ ml) and A549 (10.1µg/ml) cell lines. In vivo study indicated that both complexes produced remarkable antitumor activity against EAC cells inoculated to mice, however, Co- complex showed powerful activity than Cr- complex. Increased life span of mice bearing tumor and decreased ascetic fluid volume in addition to decreased percent of cell viability were noted post cobalt complex treatment compared to post chromium treatment. Hematological profile of EAC inoculated mice reverted to better values in case of treatment with Co- complex compared to Cr- complex. Both complexes had ameliorated the changes in liver function exerted by EAC inoculation. Moreover, post Co- complex treatment significantly improved the antioxidant status of EAC bearing mice, however Cr- complex failed. Results of biochemical study including liver, kidney and heart functions were further supported by histopathological examination of these vital organs. It could be concluded that cobalt complex may be a promising effective anticancer agent than chromium complex, properly through enhancing antioxidant activity of animals bearing tumor. |
|
Supervisor |
: |
Prof. Amani Farouk Hussein Noureldeen |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1438 AH
2017 AD |
|
Co-Supervisor |
: |
prof. Safaa Yousef Qusti |
|
Added Date |
: |
Wednesday, January 25, 2017 |
|
Researchers
| وفاء عبدالله العمودي | Al-Amoudi, Wafa Abdullah | Researcher | Master | |
|